Creative Peptides provides PNA FISH probes with high specificity and sensitivity to the target centromere sequence.
Browse our catalog below to find your products of interest.
|CP-017||CENPB-RC-Cy3||Cy3 labeled reverse complement of CENPB probe||5 nmole|
|CP-018||CENT-RC-Alexa488||Alexa 488 labeled reverse complement of CENT probe||5 nmole|
What is PNA
Protein, the material basis of life, consists of 20 amino acids arranged in peptide bonds; nucleic acid, the genetic material of life, consists of 4 nucleotides arranged in phosphodiester bonds. In 1991, four Danish biochemists, Dr. Nielsen, Dr. Egholm, Dr. Berg and Dr. Buchardt ingeniously fused the two to create a peptide nucleic acid molecule (PNA).
PNA is a synthetic deoxyribonucleic acid (DNA) analogue that retains the bases and deoxyribose of DNA in its structure, while the original phosphodiester bond backbone is replaced by the peptide bond backbone of proteins. Hence, PNA retains the base complementary pairing function, while the backbone is converted from negative to nearly neutral electrical properties. Correspondingly, PNA, when complementary to DNA, attenuates the homophilic repulsion between double-stranded DNA and is immune to nuclease degradation, gaining additional stability.
Fig. 1 Structure of DNA and PNA.
Why is PNA Probe
- PNA has better stability than DNA.
- PNA has a stronger affinity for hybridization with DNA.
- Fast hybridization rate between PNA and DNA.
- PNA and DNA hybridization environment are independent of salt ion.
- Generally DNA probes are 20-30 nt in length and anneal at around 54°C, while PNA probes are only 13-18 nt in length and can be annealed at room temperature, which naturally improves the sensitivity of the captured template.
Centromere FISH Probes
Based on PNA's specificity and stability, PNA fluorescence in situ hybridization (FISH) probes can confirm chromosome number abnormalities such as trisomy, polychromosomes and chromosome breaks by detecting chromosomal tautomers. Meanwhile, DNA or RNA probes' efficiency limits their application in detecting telomere repeat lengths. In contrast, PNA can complete hybridization rapidly, effectively reducing background noise and providing accurate information on each chromosome's telomere length.
Applications of Centromere FISH Probes
- Detect chromosomal haploidy or aneuploidy
- As a reference probe for other genes
- Eva Mateo-Mart; et al. A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques. InTech. 2010.
For Research Use Only. Not For Clinical Use.