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Centromere Probes

Centromere Probes

Creative Peptides' PNA Centromere Probes are highly specific, reliable and reusable primers.

Browse our catalog below to find your products of interest.

Structure Product Name / CAS / Cat Description / Size Price
CENPB-Alexa488 CAS:CP-001
Catalog:CP-001
Size:5 nmol
CENT-Alexa488 CAS:CP-002
Catalog:CP-002
Size:5 nmol
CENPB-Alexa647 CAS:CP-003
Catalog:CP-003
Size:5 nmol
CENT-Alexa647 CAS:CP-004
Catalog:CP-004
Size:5 nmol
CENPB-Biotin CAS:CP-005
Catalog:CP-005
Size:5 nmol
CENT-Biotin CAS:CP-006
Catalog:CP-006
Size:5 nmol
CENT-Cy3 CAS:CP-007
Catalog:CP-007
Size:5 nmol
CENPB-Cy3 CAS:CP-008
Catalog:CP-008
Size:5 nmol
CENT-Cy5 CAS:CP-009
Catalog:CP-009
Size:5 nmol
CENPB-Cy5 CAS:CP-010
Catalog:CP-010
Size:5 nmol
CENT-FAM CAS:CP-011
Catalog:CP-011
Size:5 nmol
CENPB-FAM CAS:CP-012
Catalog:CP-012
Size:5 nmol
CENT-FITC CAS:CP-013
Catalog:CP-013
Size:5 nmol
CENPB-FITC CAS:CP-014
Catalog:CP-014
Size:5 nmol
CENT-TAMRA CAS:CP-015
Catalog:CP-015
Size:5 nmol
CENPB-TAMRA CAS:CP-016
Catalog:CP-016
Size:5 nmol
CENPB-RC-Cy3 CAS:CP-017
Catalog:CP-017
Cy3 labeled reverse complement of CENPB probe Size:5 nmole
CENT-RC-Alexa488 CAS:CP-018
Catalog:CP-018
Alexa 488 labeled reverse complement of CENT probe Size:5 nmole

Introduction

What is PNA

Protein, the material basis of life, consists of 20 amino acids arranged in peptide bonds; nucleic acid, the genetic material of life, consists of 4 nucleotides arranged in phosphodiester bonds. In 1991, four Danish biochemists, Dr. Nielsen, Dr. Egholm, Dr. Berg and Dr. Buchardt ingeniously fused the two to create a peptide nucleic acid molecule (PNA).

PNA is a synthetic deoxyribonucleic acid (DNA) analogue that retains the bases and deoxyribose of DNA in its structure, while the original phosphodiester bond backbone is replaced by the peptide bond backbone of proteins. Hence, PNA retains the base complementary pairing function, while the backbone is converted from negative to nearly neutral electrical properties. Correspondingly, PNA, when complementary to DNA, attenuates the homophilic repulsion between double-stranded DNA and is immune to nuclease degradation, gaining additional stability.

Structure of DNA and PNA. Fig. 1 Structure of DNA and PNA.

Why is PNA Probe

  • PNA has better stability than DNA.
  • PNA has a stronger affinity for hybridization with DNA.
  • Fast hybridization rate between PNA and DNA.
  • PNA and DNA hybridization environment are independent of salt ion.
  • Generally DNA probes are 20-30 nt in length and anneal at around 54°C, while PNA probes are only 13-18 nt in length and can be annealed at room temperature, which naturally improves the sensitivity of the captured template.

Centromere FISH Probes

Based on PNA's specificity and stability, PNA fluorescence in situ hybridization (FISH) probes can confirm chromosome number abnormalities such as trisomy, polychromosomes and chromosome breaks by detecting chromosomal tautomers. Meanwhile, DNA or RNA probes' efficiency limits their application in detecting telomere repeat lengths. In contrast, PNA can complete hybridization rapidly, effectively reducing background noise and providing accurate information on each chromosome's telomere length.

Applications of Centromere FISH Probes

  • Detect chromosomal haploidy or aneuploidy
  • As a reference probe for other genes

Reference

  • Eva Mateo-Mart; et al. A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques. InTech. 2010.

For Research Use Only. Not For Clinical Use.