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Centromere Probes

Centromere Probes

Creative Peptides provides PNA FISH probes with high specificity and sensitivity to the target centromere sequence.

Browse our catalog below to find your products of interest.

Structure Product Name / CAS / Cat Description / Size Price
CENPB-Alexa488

Catalog:CP-001



Size:5 nmol
CENT-Alexa488

Catalog:CP-002



Size:5 nmol
CENPB-Alexa647

Catalog:CP-003



Size:5 nmol
CENT-Alexa647

Catalog:CP-004



Size:5 nmol
CENPB-Biotin

Catalog:CP-005



Size:5 nmol
CENT-Biotin

Catalog:CP-006



Size:5 nmol
CENT-Cy3

Catalog:CP-007



Size:5 nmol
CENPB-Cy3

Catalog:CP-008



Size:5 nmol
CENT-Cy5

Catalog:CP-009



Size:5 nmol
CENPB-Cy5

Catalog:CP-010



Size:5 nmol
CENT-FAM

Catalog:CP-011



Size:5 nmol
CENPB-FAM

Catalog:CP-012



Size:5 nmol
CENT-FITC

Catalog:CP-013



Size:5 nmol
CENPB-FITC

Catalog:CP-014



Size:5 nmol
CENT-TAMRA

Catalog:CP-015



Size:5 nmol
CENPB-TAMRA

Catalog:CP-016



Size:5 nmol
CENPB-RC-Cy3

Catalog:CP-017
Cy3 labeled reverse complement of CENPB probe


Size:5 nmole
CENT-RC-Alexa488

Catalog:CP-018
Alexa 488 labeled reverse complement of CENT probe


Size:5 nmole

Introduction

What is PNA

Protein, the material basis of life, consists of 20 amino acids arranged in peptide bonds; nucleic acid, the genetic material of life, consists of 4 nucleotides arranged in phosphodiester bonds. In 1991, four Danish biochemists, Dr. Nielsen, Dr. Egholm, Dr. Berg and Dr. Buchardt ingeniously fused the two to create a peptide nucleic acid molecule (PNA).

PNA is a synthetic deoxyribonucleic acid (DNA) analogue that retains the bases and deoxyribose of DNA in its structure, while the original phosphodiester bond backbone is replaced by the peptide bond backbone of proteins. Hence, PNA retains the base complementary pairing function, while the backbone is converted from negative to nearly neutral electrical properties. Correspondingly, PNA, when complementary to DNA, attenuates the homophilic repulsion between double-stranded DNA and is immune to nuclease degradation, gaining additional stability.

Structure of DNA and PNA. Fig. 1 Structure of DNA and PNA.

Why is PNA Probe

  • PNA has better stability than DNA.
  • PNA has a stronger affinity for hybridization with DNA.
  • Fast hybridization rate between PNA and DNA.
  • PNA and DNA hybridization environment are independent of salt ion.
  • Generally DNA probes are 20-30 nt in length and anneal at around 54°C, while PNA probes are only 13-18 nt in length and can be annealed at room temperature, which naturally improves the sensitivity of the captured template.

Centromere FISH Probes

Based on PNA's specificity and stability, PNA fluorescence in situ hybridization (FISH) probes can confirm chromosome number abnormalities such as trisomy, polychromosomes and chromosome breaks by detecting chromosomal tautomers. Meanwhile, DNA or RNA probes' efficiency limits their application in detecting telomere repeat lengths. In contrast, PNA can complete hybridization rapidly, effectively reducing background noise and providing accurate information on each chromosome's telomere length.

Applications of Centromere FISH Probes

  • Detect chromosomal haploidy or aneuploidy
  • As a reference probe for other genes

Reference

  • Eva Mateo-Mart; et al. A Novel Type of Nucleic Acid-based Biosensors: the Use of PNA Probes, Associated with Surface Science and Electrochemical Detection Techniques. InTech. 2010.

For Research Use Only. Not For Clinical Use.