miRNA Inhibitors

miRNA Inhibitors

Creative Peptides provides high-quality miRNA Inhibitors for establishing the foundation of miRNA research and helping researchers achieve precise targets.

Browse our catalog below to find your products of interest.

Introduction

What is miRNA inhibitor

miRNA inhibitor is a chemically modified RNA single strand, which can be competitively combined with mature miRNA sequences. It is easy to obtain, simple to operat, and has a short experimental period, which can be transfected into cells by wrapping it with transfection reagent, without the tedious operation of vector construction and the concern of virus protection. Transfection efficiency can be observed by transfection control.

Mechanism

Inhibition of miRNA using antisense oligonucleotides is a unique and effective technique for characterizing and subsequently targeting miRNA function. Chemical modification of miRNA inhibitors is necessary not only to improve its affinity to the target miRNA, but also to protect miRNA inhibitors from nuclease degradation. Recent studies have described many of these modified miRNA inhibitors, including peptide nucleic acid (PNA), locked nucleic acid (LNA), and 2'-O-methyl and 2'-O-methoxyethyl modified oligonucleotides. PNA is an artificial oligonucleotide constructed on a peptide-like skeleton. PNA can forcibly prevent miRNAs from binding to the 3'-untranslated region(UTR) of their target mRNAs attenuate gene silencing effect of endogenous miRNA and increase protein expression. Compared with natural nucleic acids, PNA has a stronger affinity and higher specificity for DNA or RNA, and is resistant to nucleases. In addition, studies have shown that the presence of non-terminal mercaptan groups, such as Cys residues, greatly enhances PNA activity due to better cellular uptake.

Advantages of PNA as miRNA Inhibitors

• Higher inhibition efficiency
• Strong stability, not easy to be degraded by ribozyme or protease
• Strong specificity, not easy to miss the target
• Low cytotoxicity
• The modified peptide nucleic acid monomer is more stable and can be preserved for a long time at 4℃

Applications of miRNA Inhibitors

• Inhibition of miRNA activity in vivo to study the effect of loss-of-function
• Screening miRNA that regulates gene expression and influences cell development
• To study the role of miRNA in biological processes, such as cell development, proliferation, differentiation and apoptosis
• Find and verify endogenous miRNA targets

References

• Su Y O; et al. A highly effective and long-lasting inhibition of miRNAs with PNA-based antisense oligonucleotides. Molecules & Cells. 2009, 28(4): 341.
• Torres A G; et al. Chemical structure requirements and cellular targeting of microRNA-122 by peptide nucleic acids anti-miRs. Nucleic Acids Research. 2012(5): 2152-2167.
• Oh S Y; et al. PNA-based antisense oligonucleotides for micrornas inhibition in the absence of a transfection reagent. Oligonucleotides. 2010, 20(5): 225-230.
• Ivanova G D; et al. Improved cell-penetrating peptide-PNA conjugates for splicing redirection in HeLa cells and exon skipping in mdx mouse muscle. Nucleic Acids Research. 2008, 36(20): 6418-28.
• Shiraishi T; et al. Calcium ions effectively enhance the effect of antisense peptide nucleic acids conjugated to cationic tat and oligoarginine peptides. Cell Chemical Biology. 2005, 12(8): 923-929.

For Research Use Only. Not For Clinical Use.