Creative Peptides provides PNA FISH probes with high specificity and sensitivity to target telomere sequences. Click here to learn more!
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|TP-017||Telomere PNA Kit||20 tests|
|TP-018||aTelC-Alexa488||Alexa Fluor 488 labeled TelC probe||5 nmole|
Fluorescence in situ hybridization (FISH) is an important non-radioactive in situ hybridization technology. It follows the principle of complementary base pairing. Probes containing fluorescent substances are spliced to the target DNA by special means. Finally, the position of target DNA can be directly observed by fluorescence microscopy. Telomere is a specialized structure consisting of multiple, short repetitive non-transcribed sequences (TTAGGG) and binding proteins. Telomeres are present at the ends of linear chromosomes in eukaryotes. All known eukaryotic telomeres contain simple repeats rich in G and C complementary strands. These telomeric repeats have a general structure (T or A)m(G)n.
- Telomeres protect chromosomes from degradation and DNA repair activities.
- Telomeres provide a buffer for non-transcribed DNA outside.
- It is involved in chromosome positioning, replication, protection and control of cell growth and lifespan.
- It is closely related to apoptosis, cell transformation and immortalization.
Repeated sequences in telomeres are useful candidates for synthetic telomere FISH probes, also known as TeloFISH probes.
Peptide nucleic acid (PNA) is a novel DNA analogue. PNA can recognize and bind DNA or RNA sequences to form a stable double helix structure.
PNA probes have additional advantages compared to DNA probes (Fig. 1).
Fig. 1 Parameter comparison of DNA and PNA.
PNAs are very effective as FISH probes, even at very low concentrations. PNA probe binds to the target sequence rapidly (within a few hours) with much less interfering background.
TelG is a G-rich telomeric probe designed to detect lagging strands (TTAGGG repeats).
TelC is a C-rich telomere probe designed to detect leading strands (TAACCC repeat).
Both TelC and TelG PNA telomere probes are apply to rat chromosomes, human and mouse.
For Research Use Only. Not For Clinical Use.