Co-transfection with DNA Strategy for PNA Delivery
Co-transfection of DNA is a technique increasingly used by biological researchers. Commonly used applications of co-transfection are virus production, protein-protein interaction research, etc. Among them, the PNA-DNA hybrid cationic liposome transfection method can effectively increase the uptake of PNA by cells.
Transfection is the process of artificially introducing nucleic acid into cells by means other than viral infection. Various chemical, biological, or physical methods are used to introduce exogenous nucleic acids, resulting in changes in cell characteristics. All transfection strategies can be roughly divided into two types:
- Transient transfection. The introduced nucleic acid exists in the cell for a limited time.
- Stable transfection. Allows long-term maintenance of the foreign nucleic acid in the transfected cells and their progeny.
Co-transfection refers to the simultaneous transfection of two independent nucleic acid molecules, such as DNA and PNA. Co-transfection is a common procedure for stable transfection.
PNA and Complementary DNA Co-transfection Protocol
- The PNA is reversely complementary to the target sequence nucleotide and is fluorescently labeled, and the 3'end of the DNA is complementary to the 5'end of the PNA. The sequence and complementation are made according to the actual situation.
- The cells can be provided by the customer or directly by the Creative Peptides .
- The equal amount of PNA and DNA solution is annealed in the PCR machine to form a combination of PNA and DNA. When the cells are cultured to a cell confluency of 30% to 50%, a cationic liposome-mediated transfection test is performed, and the cells are collected at different times for related testing.
- Use flow cytometry to detect PNA transfection effect.
- Detect the effect of PNA on cell gene expression by RT-PCR.
Transfection reagent protocol
Our reagent program is for a variety of cell types and can optimize the efficiency and viability of transfection, especially when the transfection target is a new cell, we can develop a specific cell plan for you to further optimize.
Stable transfection solutions
Factors such as the amount and ratio of DNA, incubation time, and cell density are the keys to the success of transfection. For each cell type and vector, we systematically examine these factors and maintain stable transfection to help ensure reproducible results.
- We optimize the transfection conditions to the greatest extent, and select the appropriate transfection protocol for the cell type to improve transfection efficiency.
- Creative Peptides provides general guidelines for co-transfection with DNA to maximize the transfection efficiency of many commonly used cell types and help customers achieve the best results.
- When encountering transfection problems, we will analyze possible causes for you and provide suggested solutions.
Creative Peptides can provide unique co-transfection reagents and co-transfection solutions with DNA, using our advanced technology to achieve excellent transfection performance, and provide excellent transfection efficiency for a wide range of PNA cell delivery types. Please contact us to learn about the most effective PNA and DNA co-transfection strategy to choose the solution that suits you.
Notice: Products and services are used only for scientific research.