Antibacterial PNA Targeting Essential Gene mRNA Development
PNA is a completely new chemical entity that is not available in nature. Bacteria do not have the inherent ability to resist PNA. Moreover, there is no known enzyme that can degrade PNA, so it is impossible for bacteria to produce enzymes that can remove PNA.
Creative Peptides is equipped with the leading antibacterial PNA discovery technology platform, and the laboratory team has rich experience in antisense PNA discovery of bacterial diseases. Through our system services, customers can speed up their bacterial disease research while reducing project cost and time.
mRNA of Essential Genes as Target for Antisense PNA
For bacteria, it is impossible to generate mutations in their highly conserved genome sequence regions. Therefore, the use of PNA drugs targeting this sequence region may become a new antibacterial drug, and it is almost impossible for bacteria to develop resistance mechanisms to such drugs.
In past studies, the mRNA encoding of essential genes in many pathogens has been verified as possible targets for antisense PNA, the most effective is the PNA that is complementary to the mRNA around the start codon and its adjacent region. By binding to mRNA, PNA acts as a steric hindrance, which is different from some other oligonucleotides that induce RNase H activity.
Antibacterial PNA Targeting Essential Gene mRNA Development
Here are some verified PNA target genes and strains and the minimal inhibitory concentration (MIC) required for the growth of known bacteria, which can be used as a reference.
Table.1 Development of PNA targeting essential genes of bacteria (Wojciechowska M.; et al.2020)
Target | Function | Bacteria | MIC (μM) |
|---|---|---|---|
acpP | Fatty acid biosynthesis | Escherichia coliK-12 | 0.6 |
hmrB | Fatty acid biosynthesis | Staphylococcus aureusRN4220 | 10 |
folA | Folate biosynthesis | Escherichia coli AS19 | 2.5 |
folP | Folate biosynthesis | Escherichia coli AS19 | 2.5 |
gyrA | DNA replication | Klebsiella pneumoniae | 20 |
rpoD | DNA transcription | Escherichia coli(ESBL+) | 6.2 |
murA | Cell-wall biogenesis | Escherichia coliDH10B | 2.4 |
ftsZ | Cell division | Bacillus subtilis168 | 4 |
Our Services
- Customize multiple strategies for different goals. A wide range of targets include multidrug-resistant (MDR) bacteria, many other gram-negative and gram-positive bacteria, mycoplasma and chlamydia, etc.
- A professional PNA development platform has been established to develop biomolecules against bacterial infections. Our scientists have adopted different techniques and different strategies to discover antibacterial PNA.
- Our team includes highly professional PhD-level scientists, and our services have competitive and affordable prices.
Creative Peptides is a long-term expert in the discovery of antimicrobial PNA in the field of bacterial diseases. We have completed many projects and successfully obtained high-quality antibacterial PNA with high affinity and specificity. We provide custom services according to customer needs, please contact us for more information and detailed quotations.
Reference
- Wojciechowska M.; et al.Antibacterial peptide nucleic acids—facts and perspectives. Molecules. 2020, 25(3):559.
Notice: Products and services are used only for scientific research.
