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Modification of Pyrimidine Bases

Modification of Pyrimidine Bases

In addition to modifying the PNA backbone, people have also modified the base part of the PNA to improve the biological activity of the modified PNA, in order to expand the application prospects and specificity of PNA. In the structural modification of PNA, there are few types of base modification, mainly the introduction of monomers composed of pseudocytosine, in order to make the modified peptide nucleic acid and DNA form a triple helix without pH dependence.

Modification of Pyrimidine Bases

Creative Peptides has provided many years of professional support to local, domestic and international customers. In addition, we have established long-term cooperative relationships with many organizations and provide them with PNA technical services or customized services for PNA synthesis and modification. The quality and timeliness of our work and our communication skills have won praise from our customers.

Modification Examples

Here are some common examples of pyrimidine base modifications.

Pyrimidine base type

Application

Structure

2-Thiouracil

For the development of pseudo-complementary PNA

The structure of modification of nucleobases in PNA monomers

Pseudoisocytosine

For stable triplex formation with RNA duplexes

The structure of modification of nucleobases in PNA monomers

Thio-pseudoisocytosine

For stable triplex formation with RNA duplexes

The structure of modification of nucleobases in PNA monomers

2-Aminopyrimidine

For stable triplex formation with RNA duplexes

The structure of modification of nucleobases in PNA monomers

N4-benzoylcytosine

For a pseudo-complementary G-C base pair

The structure of modification of nucleobases in PNA monomers

5-(Acridin-9-ylamino)uracil

As fluorescent,hydrolytically labile nucleobase modification

The structure of modification of nucleobases in PNA monomers

Pyrene-labeled

To investigate stacking interactions and selective excimer emission in PNA2/DNA triplexes

The structure of modification of nucleobases in PNA monomers

2-Pyrimidinone

Bound strongly to a homopurine tract of complementary RNA

The structure of modification of nucleobases in PNA monomers

Furan-modified uracil derivative

As a mildly inducible, irreversible inter-strand crosslinking system targeting single and double-stranded DNA

The structure of modification of nucleobases in PNA monomers

Highlights

  • They have strong sequence stability when hybridizing PNA with DNA and RNA, can even form a stable triplex with DNA or RNA duplex. Some still hybridize to DNA or RNA through Watson-Crick base pairing.
  • The modified PNA monomer has enhanced binding affinity when hybridized with DNA or RNA.
  • Some bases have fluorescent properties and do not require separate fluorescent labels.
  • Some bases can also improve the photochemical properties of PNA.

Our Services

Modification of Pyrimidine Bases
  • PNA related knowledge and technical consultation; help customers solve problems encountered in research and promote the progress of customer research.
  • Consulting on custom synthesis and modification of PNA; consulting on selecting appropriate designs and methods to improve the efficiency and quality of services required by customers.
  • Identification and analysis of PNA samples; through a strict purification and identification process, the high purity of the PNA we provide is guaranteed.

As a research organization that provides full-service PNA services, Creative Peptides' customer-centric collaborative approach helps us build true partnerships with customers. If you want to find a reliable partner to participate in your development plan, please feel free to contact us.

Reference

  • Wojciechowska, M.et al.Antibacterial peptide nucleic Acids—Facts and perspectives. Molecules.2020, 25(3), 559.

Notice: Products and services are used only for scientific research.