Modification of Pyrimidine Bases
In addition to modifying the PNA backbone, people have also modified the base part of the PNA to improve the biological activity of the modified PNA, in order to expand the application prospects and specificity of PNA. In the structural modification of PNA, there are few types of base modification, mainly the introduction of monomers composed of pseudocytosine, in order to make the modified peptide nucleic acid and DNA form a triple helix without pH dependence.
Creative Peptides has provided many years of professional support to local, domestic and international customers. In addition, we have established long-term cooperative relationships with many organizations and provide them with PNA technical services or customized services for PNA synthesis and modification. The quality and timeliness of our work and our communication skills have won praise from our customers.
Modification Examples
Here are some common examples of pyrimidine base modifications.
Pyrimidine base type | Application | Structure |
|---|---|---|
2-Thiouracil | For the development of pseudo-complementary PNA |  |
Pseudoisocytosine | For stable triplex formation with RNA duplexes |  |
Thio-pseudoisocytosine | For stable triplex formation with RNA duplexes |  |
2-Aminopyrimidine | For stable triplex formation with RNA duplexes |  |
N4-benzoylcytosine | For a pseudo-complementary G-C base pair |  |
5-(Acridin-9-ylamino)uracil | As fluorescent，hydrolytically labile nucleobase modification |  |
Pyrene-labeled | To investigate stacking interactions and selective excimer emission in PNA2/DNA triplexes |  |
2-Pyrimidinone | Bound strongly to a homopurine tract of complementary RNA |  |
Furan-modified uracil derivative | As a mildly inducible, irreversible inter-strand crosslinking system targeting single and double-stranded DNA |  |
Highlights
- They have strong sequence stability when hybridizing PNA with DNA and RNA, can even form a stable triplex with DNA or RNA duplex. Some still hybridize to DNA or RNA through Watson-Crick base pairing.
- The modified PNA monomer has enhanced binding affinity when hybridized with DNA or RNA.
- Some bases have fluorescent properties and do not require separate fluorescent labels.
- Some bases can also improve the photochemical properties of PNA.
Our Services
- PNA related knowledge and technical consultation; help customers solve problems encountered in research and promote the progress of customer research.
- Consulting on custom synthesis and modification of PNA; consulting on selecting appropriate designs and methods to improve the efficiency and quality of services required by customers.
- Identification and analysis of PNA samples; through a strict purification and identification process, the high purity of the PNA we provide is guaranteed.
As a research organization that provides full-service PNA services, Creative Peptides' customer-centric collaborative approach helps us build true partnerships with customers. If you want to find a reliable partner to participate in your development plan, please feel free to contact us.
Reference
- Wojciechowska, M.et al.Antibacterial peptide nucleic Acids—Facts and perspectives. Molecules.2020, 25(3), 559.
Notice: Products and services are used only for scientific research.
