PNA-Based Optical Biosensors Development

PNA-Based Optical Biosensors Development

Optical biosensors have the advantages of good selectivity, low cost, high sensitivity, short analysis time, and real-time monitoring in complex biological systems. They are of great significance in the fields of biology, medicine, chemistry, food safety, and environmental monitoring. Creative Peptides provides highly sensitive and selective optical biosensors based on PNA probe technology.

The Importance of PNA in Optical Biosensors Development

The optical biosensor is composed of bioreceptors, signal sensors, and signal amplifiers. The application of PNA as a bioreceptor is of great significance to the development of biosensors.

  • PNA improves the selectivity and specificity of biosensors due to its unique physical and chemical properties. Using PNA as a probe is beneficial to the development of optical biosensors.
  • The chemical and thermal stability of PNA and the specific interaction with the sensor surface make PNA the best probe molecule for the development of different types of affinity biosensors.

PNA Applications in Optical Biosensors Development

Optical biosensors have obvious advantages of real-time, label-free and direct measurement. Here is a summary of PNA-based optical biosensors for detecting various analytes for reference.

Table1. PNA-based optical biosensors (Saadati A.; et al. 2019)



Limit of detection



1.0 × 10−12mol/L


16s rRNA

58.2 ± 1.37 pg/mL

Localized SPR (LSPR)

E542K, E545K, methylation in PIK3CA gene

50 fM

Microstructured optical fiber (MOF)

Genomic DNA



DNA hybridization

1 fM


DNA (PCR) encoding the verotoxin 2 of E. coli

7.5 × 10−9mol/L

Our Solutions

There are different types of optical biosensors such as adsorption, luminescence, fluorescence, optical fiber and surface plasmon resonance (SPR), etc. Creative Peptides has conducted a number of studies on the application of PNA-based optical biosensors, and provides development solutions for PNA-based optical biosensors for detecting various analytes, including but not limited to the following.

  • Detection of miRNA

    We are able to develop different kinds of PNA-based optical biosensors, which can detect miRNAs sensitively, quickly and selectively at the clinical level, is an important tool for disease diagnosis without the need for complex and expensive tools and equipment.

  • Detection of bacteria

    We design PNA probes to develop SPR biosensors, which generate and amplify signals through specific interaction with the sensor surface. With this simple and rapid technique, no rRNA purification steps are required, and pathogens can be detected very sensitively.

  • Detection of DNA hybridization and point mutations

    Using polarized light to illuminate the interface between the sample and the sensor surface, the hybridization of PNA and DNA or RNA causes a change in refractive index. We can use this method to monitor the hybridization process in real time during the test.

Technical Advantages

Creative Peptides focuses on the research and technological development of PNA-based biosensors, mainly for the development of new optical biosensors for the healthcare, environmental applications and biotechnology industries. If you have any related ideas, please feel free to contact us to help you realize your biosensor development project.

Technical advantages - Creative Peptides Reference
  • Saadati, A.; et al. Recent advances on application of Peptide Nucleic Acids as a bioreceptor in biosensors development. TrAC Trends in Analytical Chemistry. 2019.

Notice: Products and services are used only for scientific research.