PNA Oligomer Synthesis

PNA Oligomer Synthesis

Creative Peptides team has accumulated rich experience in PNA chemistry, we can provide two chemical synthesis methods to synthesize PNA oligomers, including Boc chemistry and Fmoc chemistry. The entire synthesis process will be implemented in accordance with strict quality control protocols, and we are committed to synthesizing high-quality, high-precision, and high-activity PNA products to meet customer expectations.

About PNA Oligomer Synthesis

PNA is a pseudo-peptide backbone instead of the ribonucleic acid-phosphate backbone, retaining the original 4 bases. Nielsen et al in Rose Laboratory of the University of Copenhagen used a simple computer DNA model to successfully design and synthesize PNA and PNA in 1991. The synthesis of PNA is first to synthesize the monomers, and then oligomerize the PNA monomers according to the standard peptide synthesis method. The structural unit of PNA is modified amino acid, so it can be prepared in large quantities by peptide synthesis, and it can also be prepared by chemical synthesis by attaching functional groups to the N-terminus or C-terminus. PNA oligomers are prepared from protected PNA monomers by solid-phase synthesis, and their protection strategies, condensation conditions, deprotection methods and purification methods are similar to those of peptide synthesis.

Provide Services of Two Synthesis Routes

Synthesis of PNA Oligomers by Boc Chemistry

The Boc chemistry is a chemical method that uses TFA removable Boc (tert-butoxycarbonyl) as an α-amino protecting group, and side chain protection uses benzyl alcohol. During synthesis, a Boc amino acid derivative is covalently cross-linked to the resin, the Boc is removed with TFA, the free amino terminal is neutralized with triethylamine, and then activated by DCC to couple the next amino acid, and the final deprotection usually uses HF method or TFMSA method. Many biological macromolecules have been successfully synthesized by Boc method, such as active enzymes, growth factors, artificial proteins and so on.

Synthesis of PNA Oligomers by Fmoc Chemistry

The fundamental difference between the Fmoc chemistry and the Boc chemistry is that the alkali-removable Fmoc (9-fluorenylmethyloxycarbonyl) is used as the protective group of the α-amino group, and the side chain is protected by the tert-butoxy group which can be removed by TFA , the resin adopts 90% TFA-removable p-alkoxybenzyl alcohol resin, and the final deprotection avoids strong acid treatment.

Problems in the Synthesis of PNA

  • Components are expensive.
  • There are many difficult sites for synthesis. When the PNA polyamide framework forms an amide bond, the protecting group is prone to steric hindrance.
  • The purity of the monomer used has a great impact on the biological activity of the target PNA.

Our Advantages

  • High Quality

    Product is identified and analyzed, and HPLC and MS analysis data are provided.

  • High Purity

    Provide high-purity
    monomer raw materials to ensure the refined purity of synthetic samples.

  • High Activity

    Through the
    improvement of purity, the biological activity of the target PNA is guaranteed.

Creative Peptides is committed to becoming a reliable and professional partner you can rely on, providing you with first-class services, accelerating project progress and maximizing your project success rate. You can contact us if you have any needs, we will solve your doubts and provide detailed information.

Notice: Products and services are used only for scientific research.