Anti-hepatitis Virus PNA Development
At present, most of the drugs used to treat hepatitis viruses are nucleoside analogs and interferons. These drugs have strong drug resistance, large side effects, and poor therapeutic effects. As an antisense agent, PNA can be combined with specific target sequences to regulate the expression of target genes at the gene level, so as to achieve the purpose of treatment and reduce side effects.
Creative Peptides provides you with PNA drug development services for hepatitis virus.
About Hepatitis Virus
Hepatitis virus refers to the pathogen that causes viral hepatitis. There are six types of human hepatitis virus as shown on the left. HBV has a spherical shape with a double-layer shell structure, and the nucleic acid is double-stranded DNA. Except for HBV genetic material which is double-stranded DNA, other types of viruses are single-stranded RNA.
PNA Application in the Treatment of Hepatitis
Table1. PNA application in the treatment of hepatitis (Sun H.; et al.2020)
Application | PNA | Sequence | Target | Mechanism |
|---|---|---|---|---|
DHBV | PNA 2053 | GCAATGTAGACGTAA | Encapsidation signal ε | Inhibit the reverse transcription process |
HBV | PNA-DR | GCAGAGGTGAA | pgRNA DR sequence | Prevent reverse transcription and protein translation |
HCV | PNA 6 | TACGAGACCTCCCGGGG | IRES 314-330 | Inhibit the translation process |
Development Strategies
• DNA virus
- In the process of HBV replication, the binding site of the stem-loop structure of the pregenomic RNA (pg RNA) and the viral reverse transcriptase can be targeted, called ε signal, which plays an important role in the reverse transcription process.
- During HBV replication, it can target pg RNA, which not only participates in the translation of viral core and polymerase proteins, but also serves as a template for genomic DNA replication.
• RNA virus
- The internal ribosome entry site (IRES) plays a key role in virus translation, which can be used as a target to inhibit the virus translation process.
- For the RNA replication process after translation, select the highly conserved sequence structure to design series PNA. PNA can block the binding of RNA polymerase in the form of steric hindrance and inhibits the initiation of RNA synthesis.
Special Service
PNA is not easy to be picked up by cells, we can provide several different services to improve the cell uptake rate of PNA.
Chemical modification
The backbone and bases in the parent structure of PNA are modified separately by chemical synthesis to synthesize a series of PNA monomer analogs, so that the compound can easily penetrate the cell membrane and enter the cell to play a role.
Conjugate CPP
Conjugation of cell penetrating peptide (CPP) to PNA can increase cellular uptake. Conjugating cationic peptides and fatty acids to obtain modified CPP can further promote endosomal escape (or avoid endosomal pathways), thereby improving bioavailability.
Oligonucleotide scaffold
Oligonucleotide scaffolds can be assembled with a variety of peptide-PNAs. After PNA assembled, the endosomes of the intracellular conjugates are reduced and the cell membrane barrier is more easily penetrated, thereby increasing peptides-PNA delivery efficiency.
Creative Peptides provides our customers with a wide range of antiviral PNA drug development services. Our scientists are committed to satisfying each unique need of customers in the discovery of PNA against hepatitis virus. In order to be able to provide services efficiently and accurately, please fill out the complete inquiry form and contact us by email, and we will reply to you as soon as possible.
Reference
- Sun H.; et al.Application of peptide nucleic acid in virus detection and treatment. Chinese Journal of Bioengineering.2020, 152-159.
Notice: Products and services are used only for scientific research.
