PNA Clamp Kits

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PNA Clamp Kits

Creative Peptides' PNA Clamp Kits are effective tools for inhibiting transcriptional repressors and promoting promoter activity.

Browse our catalog below to find your products of interest.

Structure	Product Name / CAS / Cat	Description / Size	Price
	PNA Clamp KRAS Kit (v2) CAS:PC-001 Catalog:PC-001	G12, G13 Size:30 tests
	PNA Clamp KRAS Kit (v3) CAS:PC-002 Catalog:PC-002	G12, G13, Q61 Size:25 tests
	PNA Clamp KRAS Kit (plus) CAS:PC-003 Catalog:PC-003	A59, K117, A146 Size:25 tests
	PNA Clamp KRAS Kit (v4) CAS:PC-004 Catalog:PC-004	G12, G13, A59, Q61, K117, A146 Size:25 tests
	PNA Clamp NRAS Kit (v4) CAS:PC-005 Catalog:PC-005	G12, G13, A59, Q61, K117, A146 Size:25 tests
	PNA Clamp BRAF Kit CAS:PC-006 Catalog:PC-006	BRAF V600 mutation Size:50 tests
	PNA Clamp EGFR Kit CAS:PC-007 Catalog:PC-007	G619, E19 del, T790, S768, E20 in, L858, L861 Size:25 tests
	PNA Clamp PIK3CA Kit CAS:PC-008 Catalog:PC-008	E542, E545, H1047 Size:25 tests
	PNA Clamp IDH1 Kit CAS:PC-009 Catalog:PC-009	R132 mutation Size:25 tests

Introduction

PNA is a synthetic analog of DNA that replaces the ribose or deoxyribose, phosphate backbone with a polyamide chain. Peptide nucleic acid can be widely used in molecular hybridization, in situ hybridization, mutation analysis, anticancer, antiviral antisense nucleic acid research and application for pathogen and genetic disease detection. PNA Clamp kits are a type of commercial kit that use PNA Clamp technology to detect DNA mutations.

Principle of PNA Clamp Technology

Generally, most of a sample is wild-type DNA, and mutant DNA only accounts for a very small part, so it is difficult to detect the existence of mutant DNA by ordinary PCR. PNA Clamp technology means that PNA can specifically bind to wild-type DNA, so that wild-type DNA cannot be used as a template for PCR amplification; while for mutant DNA, due to base deletion or mutation, PNA cannot bind to it, so without the interference of the wild-type DNA, the mutant DNA can be easily amplified and detection.

PNA Clamp Kits Fig. 1 Principle of the PNA KRAS Mutation Detection Kits (Number K C)

Advantages

Mutation detected within a short period of time (3 hours run time, 30 minutes hands-on time)
Simple operation
The detection requires less sample (as low as 1~25ng DNA sample)
High sensitivity (detection limit<1%)
Strong stability
Long shelf life (> 1 year).
Can be used in various types of real-time PCR instruments (eg. BI-7500, ABI-7900HT, Biorad CFX-96/384, Roche Lightcycler, Qiagen RGQ etc.)
The data analysis software is simple

Application

PNA Clamp EGFR Mutation Detection Kit detects mutations so far prevalent in the EGFR gene, including T790M
PNA Clamp IDH1 Mutation Detection Kit can detect mutations at the R132 position with high sensitivity
PNA Clamp PI3K3CA Kits can detect E542, E545, H1047 mutations in P13K

References

Wittung P; et al. DNA-like double helix formed by peptide nucleic acid. Nature. 1994, 368(6471): 561-563.
Kyung H; et al. EGFR, COX2, p-AKT expression and PIK3CA mutation in distal extrahepatic bile duct carcinoma. Pathology. 2016, 48(1):35-40.
Number K C. PNA KRAS Mutation Detection Kit.
Kim J I. Analysis of an EGFR mutation by PNA clamping method in lung carcinoid tumors. Kosin Medical Journal. 2016, 30(2): 141-141.
Orum, P E; et al. Single base pair mutation analysis by PNA directed PCR clamping. Nucleic Acids Research. 1993.

For Research Use Only. Not For Clinical Use.