PNA Synthesis Reagents

PNA Synthesis Reagents

Creative Peptides' PNA spacers and solutions provide flexible and efficient solutions for a wide range of applications.

Browse our catalog below to find your products of interest.

Introduction

Peptide nucleic acids (PNA) are novel structural analogs of DNA and are effective and stable antisense and antigene agents. PNA can specifically bind to nucleic acids with high affinity and high biological stability and is not easily degraded by nucleases and proteases. The regulation of transcription and translation by PNA can effectively inhibit oncogene expression, viral gene replication and transcription, thus showing its promising application in gene therapy.

Fig. 1 Structure of PNA (Burnham M, 2004)

Pair-binding Properties of PNA

• stability. After the hybridization of PNA with target sequence DNA or RNA molecules, the melting temperature of the complexes increases accordingly.
• Non-dependence on salt concentration. The stability of the duplex structure formed by PNA/DNA hybridization is independent of the salt concentration of the medium.
• Specificity. The hybridization and binding of PNA to complementary DNA showed high specificity.

Role of PNA

• Regulates the transcriptional processes. PNA can inhibit the binding of polymerase or transcription factors to the template, thereby affecting transcription and replication without affecting the action of the polymerase itself, or it can inhibit transcription by preventing the extension of the RNA strand.
• Regulates the translation process. The PNA-RNA double helix inhibits translation initiation, the PNA2-RNA trimer structure inhibits translation elongation, and neither PNA-RNA nor PNA2-RNA trimer is a substrate for RNase H.
• Inhibits expression of reverse transcription-regulated genes. PNA can inhibit the reverse transcription of mRNA into cDNA, and the spatial hindrance effect of PNA can selectively inhibit reverse transcription without affecting translation in a certain concentration range.

Applications of PNA

• Antisense drugs
• Antigene drugs
• Gene delivery vectors
• Inducers of endogenous gene expression

PNA has become a new class of powerful tools for molecular biology research and has a wide range of applications in gene expression, regulatory PCR amplification, molecular hybridization, gene mutation analysis, telomerase length analysis, and gene diagnosis and gene therapy for genetic diseases, tumors, viral infections and other diseases. Creative Peptides provides its customers with PNA synthesis reagents consisting mainly of spacers and other solutions.

Reference

• Burnham M; et al. Antisense peptide nucleic acids in antibacterial drug discovery. Mol Ther. 2004 Oct; 10(4): 614-5.

For Research Use Only. Not For Clinical Use.