Creative Peptides provides sequence-specific PNA inhibitors mainly for RNA.
- miRNA Inhibitors
- Globin Reduction PNA
- PNA Clamp Kits
PNA specifically binds to the miRNAs that are paired with each other, thereby inhibiting the regulation of miRNA itself.
PNA can prevent the reaction of globin so that non-globin mRNA can be amplified more easily.
PNA can bind to wild-type DNA so that it cannot be amplified by PCR, and mutant DNA can be easily amplified and detected.
PNA (Peptide Nucleic Acids) is a chemical similar to DNA, RNA, designed in a computer model by Peter E. Nielsen of the University of Copenhagen. Structure determines function. The structure of PNA (Fig. 1) determines that it is tightly and strongly bound to DNA or RNA.
Fig. 1 Structure of DNA and PNA.
Characteristics of PNA
- The phosphodiester bond of DNA is a peptide bond in PNA.
- PNA has no phosphate group and is not electrically repulsive.
- The monomer of PNA is N-(2-aminoethyl)-glycine.
- PNA exhibits more stable properties over a wider pH range.
- The Tm of PNA/DNA duplexes is 20°C higher than that of normal DNA/DNA duplexes.
- Because of its special structure, it is not readily recognized by nucleases and proteases and has low degradability.
- PNA can compete with single-stranded DNA/RNA for binding and will interfere with normal life activities in the cell due to its excellent binding power.
- Without hydrophilic phosphate groups, antisense drugs like PNA is difficult to approach the target site. Chemical modifications can improve its hydrophilicity.
Applications of PNA
- Binding DNA to interfere with replication.
- Binding RNA to interfere with transcription.
- Interfering with RNA splicing.
- As a molecular probe for DNA/RNA.
Notice: Products and services are used only for scientific research.