PNA FISH Probes
Creative Peptides provides a variety of PNA probe products with high sensitivity and specific hybridization.
Our Products
- Telomere Probes
Telomere PNA probes can perform qualitative and quantitative analysis of repeated telomere sequences.
- Centromere Probes
Centromeric PNA probe can identify the centromeric region of each chromosome and detect the numerical analysis of chromosomes.
- CAG Repeat Probes
CAG repeat sequence PNA probe is used to detect and analyze the repeat sequence.
Introduction
FISH (Fluorescence in situ hybridization) is a non-radioactive molecular cytogenetic technique developed in the late 1980s based on radioactive in situ hybridization. FISH is new in situ hybridization method, formed by replacing isotope labeling with fluorescent labeling. The probe first binds to the reporter molecule and after hybridization binds to the fluorescent dye by an immunocytochemical process
The probe is a DNA, RNA or oligo sequence used to detect the presence or expression of a specific gene or transcription product. Probes can be classified as DNA, RNA, PNA and oligo probes according to their chemical composition.
PNA (Peptide nucleic acid) is a new artificial information molecule that resembles the backbone structure of a peptide chain and carries bases. The backbone of the molecule is a long chain formed by repeatedly connecting N-(2-aminoethyl)-glycines through amide bonds, and the side chain is formed by connecting the bases to the backbone of the main chain using methylene carbonyl bonds.
Fig. 1 PNA and DNA hybridization follows the principle of complementary base pairing.
Advantages of PNA Probes
- PNA is an excellent oligonucleotide substituent.
- PNA is a non-chiral, uncharged molecule.
- PNA probe avoids the hybridization instability caused by mutual charge repulsion when oligonucleotides bind to their target genes.
- The binding of PNA probes to the target genes is not easily affected by the ionic strength of the hybridization solution.
- PNA probes have a strong hybridization advantage.
- The PNA probe improves the sensitivity of the assay.
- PNA probes can more easily cross the hydrophobic core of cell membranes.
- The unique properties of PNA allow the use of shorter probes.
Applications of PNA Probes
- Genetically modified testing
- Rapid microbial detection
- Detection of Yersinia pestis
- Disease diagnosis
Notice: Products and services are used only for scientific research.
