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Fmoc PNA Monomers

Fmoc PNA Monomers

Creative Peptides provides a full range of peptide nucleic acid (PNA) products and related services. Click here to learn about PNA monomers prepared by Fmoc for the synthesis of PNA oligomers.

Browse our catalog below to find your products of interest.

Cat Product Name Description Size Price
FM-001Fmoc-A(Bhoc)-Aeg-OHPNA monomer incorporating a A nucleobase analogue through the Fmoc protecting group1 g/2 g/ 5 g/10 g/25 g
FM-002Fmoc-C(Bhoc)-Aeg-OHPNA monomer incorporating a C nucleobase analogue through the Fmoc protecting group1 g/2 g/ 5 g/10 g/25 g
FM-003Fmoc-G(Bhoc)-Aeg-OHPNA monomer incorporating a G nucleobase analogue through the Fmoc protecting group1 g/2 g/ 5 g/10 g/25 g
FM-004Fmoc-J-(Boc)-Aeg-OH1 g
FM-005Fmoc-PNA-Maleimido Monomer
FM-006Fmoc-T-Aeg-OHPNA monomer incorporating a T nucleobase analogue through the Fmoc protecting group1 g/5 g/10 g/25 g
FM-007Fmoc-PNA-U-OHPNA monomer incorporating a U nucleobase analogue through the Fmoc protecting group
FM-008G-clamp PNA Monomers
FM-009Fmoc-Aeea-OH1 g
FM-010Fmoc-PNA-A(Boc)-OH1 g/5 g
FM-011Fmoc-PNA-C(Boc)-OH1 g/5 g
FM-012Fmoc-PNA-D(tetraBhoc)-OH1 g/5 g
FM-013Fmoc-PNA-D(tetraBoc)-OH1 g/5 g/10 g/25 g
FM-014Fmoc-PNA-G(Boc)-OH1 g/5 g
FM-015Fmoc-PNA-J(Bhoc)-OH1 g/5 g
FM-016Fmoc-PNA-M(Bhoc)-OH1 g/5 g
FM-017Fmoc-PNA-M(Boc)-OH1 g/5 g

Introduction

PNA is an oligonucleotide analog prepared by conventional solid-phase peptide synthesis (SPPS) techniques. Solid-phase synthesis-based t-Boc and Fmoc schemes are widely used. The Fmoc solution enhances high-quality synthetic products while avoiding the use of highly corrosive chemicals. As a result, Fmoc SPPS has become a commercially popular synthesis technique. The Fmoc group is significantly selective for the protection of aliphatic primary amines of PNA monomers, but the protection of this group against the extra-cyclic amino groups of nucleobases A, C and G must be determined. The protecting group must provide sufficient protection during the synthesis process. In addition, it should be easily removed under dissociative conditions and must exhibit sufficient solubility for the corresponding monomers. Bhoc is the best candidate. It allows the PNA monomer to be preserved in 0.2 mol/L NMP for several weeks. Bhoc can be removed in less than a minute with clean TFA.

The labeling of PNA is accomplished with the linking of the cross-linker to the amino terminus. PNA can be labeled directly at the amino terminals. But often an arm is added between the reporter gene and the probe so as not to interfere with hybridization. The most commonly used arm is the 8-amino-3,6-dioxaoctanoic acid known as O-monomer. Fmoc-AEEA-OH is hydrophilic. It has the advantage of improving the yield of labeling reactions and increasing the solubility of PNA in aqueous solutions.

Fig. 1 Structure of Fmoc-AEEA-OH.Fig. 1 Structure of Fmoc-AEEA-OH.

Advantages of Our Products

  • High purity
  • High solubility
  • High reactivity
  • Reasonable price

Reference

  • Behrendt R; et al. Advances in Fmoc solid-phase peptide synthesis. J Pept Sci. 2016 Jan; 22(1): 4-27.

For Research Use Only. Not For Clinical Use.